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Histochemistry in Focus A Sourcebook of Techniques and Research Needs 1st Edition

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Histochemistry in Focus A Sourcebook of Techniques and Research Needs 1st Edition,8180940306,9788180940309


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Book Information

Publisher:MJP Publishers
Published In:2007
Binding Type:Paperback
Pages:pp. xii + 873, Illus. (Some Col.), Indices, References

The Title "Histochemistry in Focus A Sourcebook of Techniques and Research Needs 1st Edition" is written by K. Shyamasundari. This book was published in the year 2007. The ISBN number 8180940306|9788180940309 is assigned to the Paperback version of this title. The book displayed here is a 1st Edition edition. This book has total of pp. xii + 873 (Pages). The publisher of this title is MJP Publishers. We have about 126 other great books from this publisher. Histochemistry in Focus A Sourcebook of Techniques and Research Needs 1st Edition is currently Available with us.

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About the Book

Histochemistry, one of the cornerstones of biological and Medical training and research, provide us with fundamental information about cell, tissue and organ structure that complements the work. This Book presents a broad array of histochemical techniques to study the cell and tissue types and its constituents present in both invertebrate and vertebrate organisms. The techniques supported with laboratory observations, preparation of various media and reagents and staining techniques would be of immense help to the students and researchers in the field of lifesciences.

About the Author

Professor Koka Shyamasundari graduated from the Andhra University in 1954 with B. Sc. degree and later with B. Sc (Hons) in 1956, and M. Sc. (by research) in 1957. She began working as a demonstrator in the Department of Zoology, Andhra University, till 1958 and was later elevated to the post of Lecturer in 1959. Her work leading to Ph. D. was on amphipods, both planktonic and fouling, with emphasis on neurosecretion and histochemistry. She was elevated to the post of Reader and Professor in succeeding years. Thirty-one students have taken Ph. D. degrees under her guidance. She has published 320 papers, most of them in international journals (36 different foreign journals). Of these, four are reviews. She has contributed significantly to histochemistry and histophysiology. She is the author of eleven books for intermediate and B. Sc. courses, besides two monographs. She has presented 80 papers at both national and international conferences. She is Fellow of Zoological Society of India, Fellow of Zoological Society of Calcutta and Member of National Academy of Sciences, Allahabad. She has travelled widely to participate in international conferences. Professor Kona Hanumantha Rao graduated from the Andhra University with B. Sc. (Hons) degree in 1950 with Marine Biology as special subject. His early research was devoted to parasites of marine organisms. He obtained his M. Sc. (by research) degree in 1952 and D. Sc. in 1958. He contributed extensively to life cycle studies of trematode parasites of Visakhapatnam. He was trained in biology of helminthes while he was a Colombo plan scholar (1958–60) at University of Leeds, University of Dublin, London School of Tropical Medicine and Hygiene, and Moltano Institute of Parasitology, University of Cambridge. During this period, he became interested in the histochemistry of Mehlis gland, and established a strong school of histochemistry and histophysiology at the Department of Zoology, Andhra University. Fifty students took Ph. D. degrees under his guidance in marine biology and metazoan parasitology (histophysiology). He has published about 370 research papers and has contributed significantly to histochemistry of scleroproteins in marine animals and parasites. He is Fellow of National Academy of Sciences, Allahabad, Fellow of National Institute of Communicable Diseases, New Delhi, Fellow of Andhra Pradesh Academy of Sciences, Hyderabad, Honorary Professor of Sri Krishna Academy and is still involved in teaching M. Sc. students and helping research scholars.


1. Fixatives and Methods of Fixation-Types of Fixatives
2. Tissue Processing Dehydration
3. Clearing, Embedding
4. Sectioning, Mounting
5. Theory of Staining-Physical & Chemical Theories
6. Dye
7. Mordants Decalcification Acid & Chelating Agents
8. Decalcification for Enzymes
9. Molluscan Shell
10. Preservation
11. Preparation of Stains-Preparation of Molar & Normal Solution
12. Buffer Tables
13. Mountants-Mounting Media
14. Haematoxylin Staining
15. Alum, Iron, Tungsten, Molybdenum & Lead Haematoxylin
16. Frozen Methods-Cryostat, Freeze-Drying, Freeze Substitution
17. Carbohydrates-Polysaccharides
18. Mucopolysaccharides
19. Alcian Dyes
20. Blocking Techniques
21. Protein-Fixation, Histochemical Techniques
22. Amyloids-Histochemical Methods
23. Nucleic Acids-Histochemical Methods
24. Lipids-Solubility Test
25. Staining Methods
26. Histochemical Techniques
27. Pigments-Endogenous & Exogenous Pigments
28. Formalin, Malarial & Bile Pigments
29. Minerals-Histochemical Methods
30. Microorganisms in Sections-Bacteria, Fungi, Rickettsia, Viruses
31. Gram Staining
32. Lillie’s Quick Method
33. Capsule & Spirochaete Staining
34. Microorganisms in Smears-Acid-Fast Staining, Bacterial Spore Staining
35. Stains for Bacterial Flagella, Rickettsiae, Coxiella and Chlamydiae & Mycoplasma
36. Enzymes-Dehydration and Embedding
37. Cryostat Sectioning
38. Phosphatases
39. Esterase, Lipases
40. Connective Tissue-Elastin, Collagen & Muscle Staining
41. Neurological Studies
42. Endocrine Glands-Pancreas, Pituitary, Thyroid & Adrenal Glands
43. Haematological Studies-Haemoglobin Staining
44. Preparation of Permanent Whole Mounts of Invertebrates or their Parts-Staining Invertebrates, Protozoa
45. Microwave Histology
46. Ultrahistochemistry-Aldehyde, Formaldehyde, Osmium Fixatives
47. Techniques in Cell Biology Argentaffin and Chromaffin Cells
48. Chromosomes
49. Golgi Apparatus
50. Mitochondria
51. Methods for Special Organs-Alimentary Canal
52. Suprarenal Glands, Teeth, Lungs
53. Skin, Bone Marrow, Ovary
54. Spleen and Lymph Glands
55. Embryos, Liver, Kidney
56. Thyroid, Invertebrate Staining Methods, Mast Cells
57. Labels
58. Immunostaining
59. Antigen Retrieval Techniques