Summary : Histochemistry in Focus A Sourcebook of Techniques and Research Needs |
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Histochemistry, one of the cornerstones of Biological and Medical training and research, provide us with fundamental information about cell, Tissue and organ structure that complements the work. This Book presents a broad array of histochemical techniques to Study the cell and tissue types and its constituents present in both invertebrate and vertebrate organisms. The techniques supported with laboratory observations, preparation of various media and reagents and staining techniques would be of immense help to the students and researchers in the field of lifesciences.
1. Fixatives and Methods of Fixation-Types of Fixatives
2. Tissue Processing Dehydration
3. Clearing, Embedding
4. Sectioning, Mounting
5. Theory of Staining-Physical & Chemical Theories
6. Dye
7. Mordants Decalcification Acid & Chelating Agents
8. Decalcification for Enzymes
9. Molluscan Shell
10. Preservation
11. Preparation of Stains-Preparation of Molar & Normal Solution
12. Buffer Tables
13. Mountants-Mounting Media
14. Haematoxylin Staining
15. Alum, Iron, Tungsten, Molybdenum & Lead Haematoxylin
16. Frozen Methods-Cryostat, Freeze-Drying, Freeze Substitution
17. Carbohydrates-Polysaccharides
18. Mucopolysaccharides
19. Alcian Dyes
20. Blocking Techniques
21. Protein-Fixation, Histochemical Techniques
22. Amyloids-Histochemical Methods
23. Nucleic Acids-Histochemical Methods
24. Lipids-Solubility Test
25. Staining Methods
26. Histochemical Techniques
27. Pigments-Endogenous & Exogenous Pigments
28. Formalin, Malarial & Bile Pigments
29. Minerals-Histochemical Methods
30. Microorganisms in Sections-Bacteria, Fungi, Rickettsia, Viruses
31. Gram Staining
32. Lillie’s Quick Method
33. Capsule & Spirochaete Staining
34. Microorganisms in Smears-Acid-Fast Staining, Bacterial Spore Staining
35. Stains for Bacterial Flagella, Rickettsiae, Coxiella and Chlamydiae & Mycoplasma
36. Enzymes-Dehydration and Embedding
37. Cryostat Sectioning
38. Phosphatases
39. Esterase, Lipases
40. Connective Tissue-Elastin, Collagen & Muscle Staining
41. Neurological Studies
42. Endocrine Glands-Pancreas, Pituitary, Thyroid & Adrenal Glands
43. Haematological Studies-Haemoglobin Staining
44. Preparation of Permanent Whole Mounts of Invertebrates or their Parts-Staining Invertebrates, Protozoa
45. Microwave Histology
46. Ultrahistochemistry-Aldehyde, Formaldehyde, Osmium Fixatives
47. Techniques in Cell Biology Argentaffin and Chromaffin Cells
48. Chromosomes
49. Golgi Apparatus
50. Mitochondria
51. Methods for Special Organs-Alimentary Canal
52. Suprarenal Glands, Teeth, Lungs
53. Skin, Bone Marrow, Ovary
54. Spleen and Lymph Glands
55. Embryos, Liver, Kidney
56. Thyroid, Invertebrate Staining Methods, Mast Cells
57. Labels
58. Immunostaining
59. Antigen Retrieval Techniques