DNA Science A First Course

This is the second Edition of a very successful textbook that combines an illustrated, Narrative text with 12 rigorously tested, reliable lab Experiments in Molecular biology, developed at the internationally renowned Dolan Dna Learning Center of Cold Spring Harbor Laboratory. Proven through more than 10 years of Teaching in Research and non-research colleges and universities, junior colleges, community colleges, and Advanced high School Biology programs, DNA Science has been successfully Integrated into introductory biology, general biology, genetics, microbiology, cell biology, molecular Genetics and molecular biology courses. The Text has been completely revised, extensively rewritten, and updated. The new coverage extends to the completion of the draft sequence of the human genome and the enormous impact these and other sequence data are having on medicine, research, and industry. The laboratory experiments cover basic techniques of gene isolation and analysis that will be successful even in the Hands of Teachers and students with no prior experience. Extensive prefab notes at the Beginning of each experiment explain how to schedule and prepare, while flowcharts and icons make the protocols easy to follow. The laboratory course is completely supported by quality-assured products from the Carolina Biological Supply Company, from bulk reagents, to usable reagent systems, to single-use kits, thus satisfying a broad range of teaching applications.

Preface

1. Chapters : How We Learned That Dna is the Genetic Material
2. How We Learned the Function of DNA
3. How We Learned how Genes are Regulated
4. Basic Tools and Techniques of DNA Science
5. Methods for Finding and Expressing Important Genes
6. Modern Methods for Analyzing Whole Genomes
7. The DNA Science of Cancer
8. Applying DNA Science to Human Genetics and Evolution
9. Laboratories: Lab Safety and Adherance to National Institute of Health Guidelines : Measurements, Micropipetting, and Sterile Techniques
10. Bacterial Culture Techniques : A. Isolation of Individual Colonies B. Overnight Suspension Culture C. Mid-log Suspension Culture
11. DNA Restriction Analysis
12. Effects of DNA Methylation on Restriction
13. Rapid Colony Transformation of E. Coli with Plasmid DNA
14. Assay for an Antibiotic Resistance Enzyme
15. Purification and Identification of Recombinant GFP : A. Plasmid Minipreparation of pAMP B. Restriction Analysis of Purified pAMP
16. Recombination of Antibiotic Resistance Genes : A. Restriction Digest of Plasmids pAMP and pKAN B. Ligation of pAMP and pKAN Restriction Fragments
17. Transformation of E. Coli with Recombinant DNA : A. Classic Procedure for Preparing Competent Cells B : Transformation of E.coli with Recombinant DNA
18. Replica Plating to Identify Mixed E.Coli Populations
19. Purification and Identification of Recombinant DNA : A. Plasmid Minpreparation of pAMP/pKAN Recombinants B. Restriction Analysis of Purified Recombinant DNA : Answers to Discussion Questions
20. APPENDICES: Equipment, Supplies, and Reagents
21 Recipes for Media, Reagents, and Stock Solutions
22. Restriction Map Data for pAMP, pKAN, pBLU, pGREEN, and Bacteriophage l
23. Cautions
24. Name Index
25. Subject Index